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conventional pcr  (Bio-Rad)


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    Structured Review

    Bio-Rad conventional pcr
    Conventional Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 14121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/conventional+pcr/T100+Thermal+Cycler/pmc12656284-232-0-9
    Average 99 stars, based on 14121 article reviews
    conventional pcr - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Effect of Fiber Drink Consumption Containing Glucomannan and Isomaltooligosaccharide on Digesta Profile and Gut Microbiota
    Article Snippet: .. Before testing using q-PCR, annealing temperature optimization was carried out using conventional PCR (Bio-Rad T100 Thermal Cycler brand produced in California, USA). .. The PCR results were then visualized using an electrophoresis device (Bio-Rad PowerPac Basic brand produced in California, USA and Hercuvan MiniGEL Electrophoresis produced in Cambridge, United Kingdom).

    Article Title: Identification of microRNA signature and potential pathway targets in prostate cancer
    Article Snippet: Total RNA (1 μg) was employed for first-strand cDNA synthesis using iScriptTM cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). .. Conventional PCR was performed using IQ SYBR Green Supermix according to manufacturer’s protocol (Bio-Rad). .. Transcript-specific primers for protein kinase B (also known as Akt ), H- ras , K- ras, c- fos , c- jun , B-cell lymphoma 2 ( Bcl2 ), ataxia telangiectasia mutated ( ATM ), and the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase were purchased from IDT.

    Article Title: Corn distiller’s dried grains with solubles as a target for fermentation to improve bioactive functionality for animal feed and as a source for a novel microorganism with antibacterial activity
    Article Snippet: 2.10 PCR with bacterial and fungal-specific primers Under aseptic conditions, a loopful of the culture isolated from pasteurized corn DDGS extracts was used in DNA extraction with the Qiagen DNeasy Plant Mini Kit (Qiagen, Cat. # 69106), and the quality of extracted DNA was determined with a UV-Vis spectrophotometer (NanoDropTM 2000; ThermoFisher). .. To determine if the growth isolated from pasteurized corn-DDGS extract (unfermented) was prokaryotic or eukaryotic, conventional PCR (C1000 Touch thermal cycler, BioRad) was performed using bacterial-specific 16s rRNA universal primers and fungal-specific ITS4/5 primers. .. The PCR was performed in 50 μL reaction volumes containing 1X Green GoTaq reaction buffer with 1.5 mM MgCl2 (Promega, Madison, WI), .2 mM of each dNTP, .4 μM of each primer, 1.25u of GoTaq DNA polymerase (Promega, Madison, WI), .5 μg of template DNA, and nuclease-free water.

    Article Title: Detection of Mycotoxigenic Fungi and Residual Mycotoxins in Cannabis Buds Following Gamma Irradiation
    Article Snippet: Sequencing libraries were prepared with NEBNext ® UltraTM DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and sequenced on an Illumina platform (Illumina, San Diego, CA, USA). .. Conventional PCR was run on a T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA), and qPCR was performed on an Mx3000P QPCR System (Agilent Technologies, Santa Clara, CA, USA). .. DNA quality and concentration were measured using a Nanodrop ND1000 spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific, Wilmington, DE, USA).

    Article Title: Detection of feline immunodeficiency virus by neutral red-based loop-mediated isothermal amplification assay.
    Article Snippet: .. Conventional PCR was performed using a T100 thermal cycler (Bio-Rad). ..

    Article Title: Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA
    Article Snippet: All the LAMP amplifications were performed at 70 °C, confirmed by gel electrophoresis using 1.5% agarose gel at 100 V for 40 min, stained with a clear vision DNA stain (PT-D1001, Pro-Tech chemicals, Taiwan), and imaged via the BluView Transilluminator (MBE-300, Major Science, USA). .. Conventional PCR (T100 thermal cycler, Bio-Rad, USA) and real-time PCR reactions (Step one plus system, Applied Biosystems) were performed with isolated plasmids using Fast-Run Taq Master Mix (PT-TMM228-D, Pro-Tech Chemicals, Taiwan) and Fast-start universal SYBR green master (rox) (4913914001, Roche, Germany), respectively. ..

    Produced:

    Article Title: Effect of Fiber Drink Consumption Containing Glucomannan and Isomaltooligosaccharide on Digesta Profile and Gut Microbiota
    Article Snippet: .. Before testing using q-PCR, annealing temperature optimization was carried out using conventional PCR (Bio-Rad T100 Thermal Cycler brand produced in California, USA). .. The PCR results were then visualized using an electrophoresis device (Bio-Rad PowerPac Basic brand produced in California, USA and Hercuvan MiniGEL Electrophoresis produced in Cambridge, United Kingdom).

    SYBR Green Assay:

    Article Title: Identification of microRNA signature and potential pathway targets in prostate cancer
    Article Snippet: Total RNA (1 μg) was employed for first-strand cDNA synthesis using iScriptTM cDNA Synthesis Kit according to the manufacturer’s protocol (Bio-Rad). .. Conventional PCR was performed using IQ SYBR Green Supermix according to manufacturer’s protocol (Bio-Rad). .. Transcript-specific primers for protein kinase B (also known as Akt ), H- ras , K- ras, c- fos , c- jun , B-cell lymphoma 2 ( Bcl2 ), ataxia telangiectasia mutated ( ATM ), and the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase were purchased from IDT.

    Article Title: Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA
    Article Snippet: All the LAMP amplifications were performed at 70 °C, confirmed by gel electrophoresis using 1.5% agarose gel at 100 V for 40 min, stained with a clear vision DNA stain (PT-D1001, Pro-Tech chemicals, Taiwan), and imaged via the BluView Transilluminator (MBE-300, Major Science, USA). .. Conventional PCR (T100 thermal cycler, Bio-Rad, USA) and real-time PCR reactions (Step one plus system, Applied Biosystems) were performed with isolated plasmids using Fast-Run Taq Master Mix (PT-TMM228-D, Pro-Tech Chemicals, Taiwan) and Fast-start universal SYBR green master (rox) (4913914001, Roche, Germany), respectively. ..

    Isolation:

    Article Title: Corn distiller’s dried grains with solubles as a target for fermentation to improve bioactive functionality for animal feed and as a source for a novel microorganism with antibacterial activity
    Article Snippet: 2.10 PCR with bacterial and fungal-specific primers Under aseptic conditions, a loopful of the culture isolated from pasteurized corn DDGS extracts was used in DNA extraction with the Qiagen DNeasy Plant Mini Kit (Qiagen, Cat. # 69106), and the quality of extracted DNA was determined with a UV-Vis spectrophotometer (NanoDropTM 2000; ThermoFisher). .. To determine if the growth isolated from pasteurized corn-DDGS extract (unfermented) was prokaryotic or eukaryotic, conventional PCR (C1000 Touch thermal cycler, BioRad) was performed using bacterial-specific 16s rRNA universal primers and fungal-specific ITS4/5 primers. .. The PCR was performed in 50 μL reaction volumes containing 1X Green GoTaq reaction buffer with 1.5 mM MgCl2 (Promega, Madison, WI), .2 mM of each dNTP, .4 μM of each primer, 1.25u of GoTaq DNA polymerase (Promega, Madison, WI), .5 μg of template DNA, and nuclease-free water.

    Article Title: Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA
    Article Snippet: All the LAMP amplifications were performed at 70 °C, confirmed by gel electrophoresis using 1.5% agarose gel at 100 V for 40 min, stained with a clear vision DNA stain (PT-D1001, Pro-Tech chemicals, Taiwan), and imaged via the BluView Transilluminator (MBE-300, Major Science, USA). .. Conventional PCR (T100 thermal cycler, Bio-Rad, USA) and real-time PCR reactions (Step one plus system, Applied Biosystems) were performed with isolated plasmids using Fast-Run Taq Master Mix (PT-TMM228-D, Pro-Tech Chemicals, Taiwan) and Fast-start universal SYBR green master (rox) (4913914001, Roche, Germany), respectively. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Detection of Mycotoxigenic Fungi and Residual Mycotoxins in Cannabis Buds Following Gamma Irradiation
    Article Snippet: Sequencing libraries were prepared with NEBNext ® UltraTM DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) and sequenced on an Illumina platform (Illumina, San Diego, CA, USA). .. Conventional PCR was run on a T100 Thermal Cycler (Bio-Rad, Hercules, CA, USA), and qPCR was performed on an Mx3000P QPCR System (Agilent Technologies, Santa Clara, CA, USA). .. DNA quality and concentration were measured using a Nanodrop ND1000 spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific, Wilmington, DE, USA).

    Article Title: Rotational diffusometric sensor with isothermal amplification for ultra-sensitive and rapid detection of SARS-CoV-2 nsp2 cDNA
    Article Snippet: All the LAMP amplifications were performed at 70 °C, confirmed by gel electrophoresis using 1.5% agarose gel at 100 V for 40 min, stained with a clear vision DNA stain (PT-D1001, Pro-Tech chemicals, Taiwan), and imaged via the BluView Transilluminator (MBE-300, Major Science, USA). .. Conventional PCR (T100 thermal cycler, Bio-Rad, USA) and real-time PCR reactions (Step one plus system, Applied Biosystems) were performed with isolated plasmids using Fast-Run Taq Master Mix (PT-TMM228-D, Pro-Tech Chemicals, Taiwan) and Fast-start universal SYBR green master (rox) (4913914001, Roche, Germany), respectively. ..



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